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Title: Comparative Analysis Of Phenolic Content And Antioxidative Activities Of Eight Malaysian Traditional Vegetables
Authors: Nur Huda-Faujan, Zulaikha Abdul Rahim
Maryam Mohamed Rehan, Faujan Bin Haji Ahmad
Keywords: Folin-Ciocalteu method, Prussian Blue assay, antioxidant activities, Malaysian vegetables
Issue Date: 2015
Publisher: Universiti Kebangsaan Malaysia
Series/Report no.: Vol 19;No 3
Abstract: Vegetables have been believed to exhibit antioxidant activities due to its phenolic content. Thus, this study was carried out to determine the total phenolic content of water and ethanolic extracts of Malaysian traditional vegetables and assess their antioxidant activities. Eight samples of Malaysian traditional vegetables were dried and extracted its phenolic compounds using water and ethanolic solvent. Total phenolic content of the extracts were compared and evaluated using Folin-Ciocalteu and Prussian Blue reagent. The antioxidant activity were assessed using ferric thiocyanate assay and DPPH free radical scavenging assays. Results found that total phenolic content of water extracts ranged from 7.08 to 14.76 mg GAE (Folin-Ciocalteu assay) and 3.50 to 7.82 mg GAE (Prussian Blue assay). However, the content of phenolic of ethanolic extracts ranged from 5.21 to 15.86 mg GAE (Folin-Ciocalteu assay), and 1.84 to 11.54 mg GAE (Prussian Blue assay). The highest antioxidant activity was observed in water extracts of Etlingera elatior (75.6%) and ethanolic extracts of Sauropus androgynus (78.1%). Results also found that the best half maximal inhibitory concentration or IC50 were demonstrated by water and ethanolic extracts of Sauropus androgynus which demonstrated 0.077 mg/mL and 0.078 mg/mL, respectively. Hence, this study obtained that most of the Malaysian traditional vegetables have a potential source of natural antioxidant.
Appears in Collections:Malaysian Journal of Analytical Sciences

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