Please use this identifier to cite or link to this item: http://ddms.usim.edu.my:80/jspui/handle/123456789/8405
Title: Silylation of mica for lipase immobilization as biocatalysts in esterification
Authors: Uswatun Hasanah, Zaidan
Mohd Basyaruddin, Abdul Rahman
Mahiran, Basri
Siti Salhah, Othman
Raja Noor Zaliha, Raja Abdul Rahman
Abu Bakar, Salleh
Keywords: Mica
Silanization
Immobilization
Candida rugosa lipase
Esterification
Issue Date: 2010
Publisher: Elsevier Science Bv
Abstract: Mica was modified either by acid treatment, grafting with aminopropyl-, octyl-, vinyl-, mercapto- and glycidoxy-triethoxysilanes, and activation of pre-treated support with glutaraldehyde (Glu). The derivatives were characterized by X-ray diffraction (XRD), infra-red spectroscopy (FTIR), surface area and porosity analysis, scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX) and transmission electron microscopy (TEM) techniques. The modified micas were used for immobilization of lipase from Candida rugosa (CRL). Activity of the lipase was determined by esterification and exhibited the improved activity than the free enzyme following the order; Amino-CRL>Glu-Amino-CRL>Octyl-CRL>Vinyl-CRL>Glycidoxy-CRL>Mercapto-CRL>Mica-CRL Lipase immobilized mica showed enhanced protein loading (up to 8.22 mg protein/g support) and immobilization (up to 78%) compared to the free lipase and unmodified mica. (C) 2009 Elsevier B.V. All rights reserved.
URI: http://ddms.usim.edu.my/handle/123456789/8405
ISSN: 0169-1317
Appears in Collections:Applied Clay Science

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